Ki-67 staining for determination of rhesus macaque T cell proliferative responses ex vivo.

ABSTRACT: The capacity for robust proliferation upon re-infection is a hallmark of adaptive immunity and the basis of vaccination. A widely used animal model for the study of human disease is the rhesus macaque (RM), where capacity for proliferation can be assessed ex vivo using carboxyfluorescein succinimidyl ester (CFSE)-based dilution assays. However, we show over the course of the standard ex vivo proliferation assay that CFSE-labeling at commonly used dye concentrations induces significant cell death, but that this phenomenon is dose-dependent. Here, we describe an alternative semiquantitative method for estimating T cell proliferative responses that avoids the putative biases associated with chemical modification. RM peripheral blood mononuclear cells were stimulated ex vivo with cognate pepti

SUBMITTER: Shedlock DJ 

PROVIDER: S-EPMC2939446 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature