Tobacco smoke induces the generation of procoagulant microvesicles from human monocytes/macrophages.

ABSTRACT:

Objective

To investigate whether exposure of human monocytes/macrophages to tobacco smoke induces their release of membrane microvesicles (MVs) that carry tissue factor (TF) released from cells, whether smoke-induced MVs are procoagulant, and what cellular processes might be responsible for their production.

Methods and results

We found that exposure of human THP-1 monocytes and primary human monocyte-derived macrophages to 3.75% tobacco smoke extract (TSE) significantly increased their total and TF-positive MV generation. More importantly, MVs released from TSE-treated human monocytes/macrophages exhibited 3 to 4 times the procoagulant activity of control MVs, as assessed by TF-dependent generation of factor Xa. Exposure to TSE increased TF mRNA and protein expression and c

SUBMITTER: Li M 

PROVIDER: S-EPMC2939448 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature