The detection of chromosomal aneusomy by fluorescence in situ hybridization in sputum predicts lung cancer incidence.

ABSTRACT: Lung cancer usually is disseminated (advanced) and has a poor prognosis at diagnosis. Current and former smokers are at a high risk for lung cancer and are candidates for prevention and early detection strategies. Sputum is a potential source of biomarkers that might determine either lung cancer risk or the presence of early lung cancer, but no current sputum test is sufficiently sensitive and specific for effective screening. We used fluorescence in situ hybridization (FISH) to measure chromosomal aneusomy (CA) in sputum samples collected prospectively from 100 incident lung cancer cases and 96 controls (matched on age, gender, and date of collection) nested within an ongoing high-risk cohort. The CA-FISH assay was aimed at four DNA targets: epidermal growth factor receptor, MYC, 5p15, an

SUBMITTER: Varella-Garcia M 

PROVIDER: S-EPMC2939746 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature