Integrated miRNA and mRNA expression profiling of the inflammatory breast cancer subtype.

ABSTRACT:

Background

MicroRNAs (miRNAs) are key regulators of gene expression. In this study, we explored whether altered miRNA expression has a prominent role in defining the inflammatory breast cancer (IBC) phenotype.

Methods

We used quantitative PCR technology to evaluate the expression of 384 miRNAs in 20 IBC and 50 non-IBC samples. To gain understanding on the biological functions deregulated by aberrant miRNA expression, we looked for direct miRNA targets by performing pair-wise correlation coefficient analysis on expression levels of 10 962 messenger RNAs (mRNAs) and by comparing these results with predicted miRNA targets from TargetScan5.1.

Results

We identified 13 miRNAs for which expression levels were able to correctly predict the nature of the sample analysed (IBC v

SUBMITTER: Van der Auwera I 

PROVIDER: S-EPMC2939785 | biostudies-literature | 2010 Aug

REPOSITORIES: biostudies-literature