Proteomics analysis of perilymph and cerebrospinal fluid in mouse.

ABSTRACT:

Objectives

Proteins in perilymph may alter the delivery profile of implantable intracochlear drug delivery systems through biofouling. Knowledge of protein composition will help anticipate interactions with delivered agents.

Study design

Analysis of mouse perilymph.

Methods

Protein composition of perilymph and cerebrospinal fluid (CSF) was analyzed using a capillary liquid chromatography-mass spectrometry-based iTRAQ quantitative proteomics approach. We searched against a mouse subset of the Uniprot FASTA protein database. We sampled perilymph from the apex of the mouse cochlea to minimize CSF contamination.

Results

More than 50 explicit protein isoforms were identified with very high confidence. iTRAQ reporter ions allowed determination of relative molar amoun

SUBMITTER: Swan EE 

PROVIDER: S-EPMC2940114 | biostudies-literature | 2009 May

REPOSITORIES: biostudies-literature