Downstream gene activation of the receptor ALX by the agonist annexin A1.

ABSTRACT:

Background

Our understanding of pro-resolution factors in determining the outcome of inflammation has recently gained ground, yet not many studies have investigated whether specific genes or patterns of genes, are modified by these mediators. Here, we have focussed on the glucocorticoid modulated pro-resolution factor annexin A1 (AnxA1), studying if its interaction with the ALX receptor would affect downstream genomic targets.

Methodology/principal findings

Using microarray technology in ALX transfected HEK293 cells, we discovered an over-lapping, yet distinct gene activation profile for AnxA1 compared to its N-terminal mimetic peptide Ac2-26, which may be suggestive of unique downstream inflammatory outcomes for each substance. When the up-regulated genes were explored, con

SUBMITTER: Renshaw D 

PROVIDER: S-EPMC2941452 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature