Distinct epigenetic domains separated by a CTCF bound insulator between the tandem genes, BLU and RASSF1A.

ABSTRACT:

Background

Tumor suppressor gene (TSG) RASSF1A and candidate TSG BLU are two tandem head-to-tail genes located at 3p21.3. We hypothesized that there may be a concordance on their gene expression and promoter methylation status. If not, then there may be an insulator located between RASSF1A and BLU genes that provides a barrier activity.

Methodology/principal findings

We first identified potential transcriptionally important CpG sites using the methylation-specific oligonucleotide array in relation to mRNA expression of RASSF1A and BLU genes in primary lung tumors. We demonstrated that E2F1 bound to the potential transcriptionally important CpG sites in RASSF1A gene of a normal lung cell line expressing RASSF1A transcripts, whereas loss of E2F1 binding to RASSF1A in A549 canc

SUBMITTER: Chang JW 

PROVIDER: S-EPMC2942851 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature