A blue spectral shift of the hemoglobin soret band correlates with the age (time since deposition) of dried bloodstains.

ABSTRACT: The ability to determine the time since deposition of a bloodstain found at a crime scene could prove invaluable to law enforcement investigators, defining the time frame in which the individual depositing the evidence was present. Although various methods of accomplishing this have been proposed, none has gained widespread use due to poor time resolution and weak age correlation. We have developed a method for the estimation of the time since deposition (TSD) of dried bloodstains using UV-VIS spectrophotometric analysis of hemoglobin (Hb) that is based upon its characteristic oxidation chemistry. A detailed study of the Hb Soret band (λmax=412 nm) in aged bloodstains revealed a blue shift (shift to shorter wavelength) as the age of the stain increases. The extent of this shift permits, fo

SUBMITTER: Hanson EK 

PROVIDER: S-EPMC2942901 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature