Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation.

ABSTRACT: Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active volume, and intracellular osmolarity have been investigated for decades, but a consistent rigorous parameterization of these concepts is lacking. Here, we subjected several data sets of minimum volume measurements in yeast obtained after hyper-osmotic shock to a thermodynamic modeling framework. We estimated parameters for several relevant biophysical cell properties and tested alternative hypotheses about these concepts using a model discrimination approach. In accordance with previous reports, we estimated an average initial turgor of 0.6 ± 0.2 MPa and found that turgor becomes negligi

SUBMITTER: Schaber J 

PROVIDER: S-EPMC2943578 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature