H-Y antibody development associates with acute rejection in female patients with male kidney transplants.

ABSTRACT:

Background

Human minor histocompatibility antigens (mHA) and clinically relevant immune responses to them have not been well defined in organ transplantation. We hypothesized that women with male kidney transplants would develop antibodies against H-Y, the mHA encoded on the Y-chromosome, in association with graft rejection.

Methods

We tested sera from 118 consecutive transplant recipients with kidney biopsies. Antibodies that specifically recognized the recombinant H-Y antigens RPS4Y1 or DDX3Y were detected by IgG enzyme-linked immunosorbent assay and western blotting. Immunogenic epitopes were further identified using overlapping H-Y antigen peptides for both the H-Y proteins.

Results

In the 26 female recipients of male kidneys, H-Y antibody development posttranspla

SUBMITTER: Tan JC 

PROVIDER: S-EPMC2943873 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature