Slug inhibits proliferation of human prostate cancer cells via downregulation of cyclin D1 expression.

ABSTRACT:

Background

Slug is a transcription factor of the Snail/Slug zinc-finger family and is implicated in metastasis of tumors, but its role in cell proliferation of prostate cancers is unclear.

Methods

Expression level of Slug and other genes was examined by Western blot, RT-PCR, and QPCR analyses. The forced expression of Slug was mediated by retroviruses and adenoviruses. Slug was downregulated by shRNA. Cell growth was measured by the MTT assay and the quick cell proliferation assay.

Results

Here, we demonstrated that Slug expression is elevated in mouse prostate tumors, and human prostate cancer cell lines LNCaP, PC-3, and 22RV1. Forced expression of Slug-inhibited proliferation of prostate cancer cells PC-3 and DU-145. Conversely, reduced expression of Slug by shRNA p

SUBMITTER: Liu J 

PROVIDER: S-EPMC2943978 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature