A systematic investigation of Escherichia coli central carbon metabolism in response to superoxide stress.

ABSTRACT:

Background

The cellular responses of bacteria to superoxide stress can be used to model adaptation to severe environmental changes. Superoxide stress promotes the excessive production of reactive oxygen species (ROS) that have detrimental effects on cell metabolic and other physiological activities. To antagonize such effects, the cell needs to regulate a range of metabolic reactions in a coordinated way, so that coherent metabolic responses are generated by the cellular metabolic reaction network as a whole. In the present study, we have used a quantitative metabolic flux analysis approach, together with measurement of gene expression and activity of key enzymes, to investigate changes in central carbon metabolism that occur in Escherichia coli in response to paraquat-induced supe

SUBMITTER: Rui B 

PROVIDER: S-EPMC2944137 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature