Local cell metrics: a novel method for analysis of cell-cell interactions.

ABSTRACT:

Background

The regulation of many cell functions is inherently linked to cell-cell contact interactions. However, effects of contact interactions among adherent cells can be difficult to detect with global summary statistics due to the localized nature and noise inherent to cell-cell interactions. The lack of informatics approaches specific for detecting cell-cell interactions is a limitation in the analysis of large sets of cell image data, including traditional and combinatorial or high-throughput studies. Here we introduce a novel histogram-based data analysis strategy, termed local cell metrics (LCMs), which addresses this shortcoming.

Results

The new LCM method is demonstrated via a study of contact inhibition of proliferation of MC3T3-E1 osteoblasts. We describe how LC

SUBMITTER: Su J 

PROVIDER: S-EPMC2944256 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature