Flexible label-free quantitative assay for antibodies to influenza virus hemagglutinins.

ABSTRACT: During the initial pandemic influenza H1N1 virus outbreak, assays such as hemagglutination inhibition and microneutralization provided important information on the relative protection afforded by the population's cross-reactivity from prior infections and immunizations with seasonal vaccines. However, these assays continue to be limited in that they are difficult to automate for high throughput, such as in pandemic situations, as well as to standardize between labs. Thus, new technologies are being sought to improve standardization, reliability, and throughput by using chemically defined reagents rather than whole cells and virions. We now report the use of a cell-free and label-free flu antibody biosensor assay (f-AbBA) for influenza research and diagnostics that utilizes recombinant hema

SUBMITTER: Carney PJ 

PROVIDER: S-EPMC2944469 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature