Expanded role for the nitrogen assimilation control protein in the response of Klebsiella pneumoniae to nitrogen stress.

ABSTRACT: Klebsiella pneumoniae is able to utilize many nitrogen sources, and the utilization of some of these nitrogen sources is dependent on the nitrogen assimilation control (NAC) protein. Seven NAC-regulated promoters have been characterized in K. pneumoniae, and nine NAC-regulated promoters have been found by microarray analysis in Escherichia coli. So far, all characterized NAC-regulated promoters have been directly related to nitrogen metabolism. We have used a genome-wide analysis of NAC binding under nitrogen limitation to identify the regions of the chromosome associated with NAC in K. pneumoniae. We found NAC associated with 99 unique regions of the chromosome under nitrogen limitation. In vitro, 84 of the 99 regions associate strongly enough with purified NAC to produce a shifted band b

SUBMITTER: Frisch RL 

PROVIDER: S-EPMC2944540 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature