Successive silencing of tandem reporter genes in potato (Solanum tuberosum) over 5 years of vegetative propagation.

ABSTRACT:

Background and aims

Transgenic plants represent an excellent tool for experimental plant biology and are an important component of modern agriculture. Fully understanding the stability of transgene expression is critical in this regard. Most changes in transgene expression occur soon after transformation and thus unwanted lines can be discarded easily; however, transgenes can be silenced long after their integration.

Methods

To study the long-term changes in transgene expression in potato (Solanum tuberosum), the activity of two reporter genes, encoding green fluorescent protein (GFP) and neomycin phosphotransferase (NPTII), was monitored in a set of 17 transgenic lines over 5 years of vegetative propagation in vitro.

Key results

A decrease in transgene expression was

SUBMITTER: Nocarova E 

PROVIDER: S-EPMC2944976 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature