BP1, an isoform of DLX4 homeoprotein, negatively regulates BRCA1 in sporadic breast cancer.

ABSTRACT:

Introduction

Several lines of evidence point to an important role for BP1, an isoform of DLX4 homeobox gene, in breast carcinogenesis and progression. BRCA1 is a well-known player in the etiology of breast cancer. While familial breast cancer is often marked by BRCA1 mutation and subsequent loss of heterozygosity, sporadic breast cancers exhibit reduced expression of wild type BRCA1, and loss of BRCA1 expression may result in tumor development and progression.

Methods

The Cister algorithm and Genomatix program were used to identify potential BP1 binding sites in BRCA1 gene. Real-time PCR, Western blot and immunohistochemistry analysis were performed to verify the expression of BRCA1 and BP1 in cell lines and breast cancer tissues. Double-stranded siRNA transfection was carri

SUBMITTER: Kluk BJ 

PROVIDER: S-EPMC2945279 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature