Plasma proteome response to severe burn injury revealed by 18O-labeled "universal" reference-based quantitative proteomics.

ABSTRACT: A burn injury represents one of the most severe forms of human trauma and is responsible for significant mortality worldwide. Here, we present the first quantitative proteomics investigation of the blood plasma proteome response to severe burn injury by comparing the plasma protein concentrations of 10 healthy control subjects with those of 15 severe burn patients at two time-points following the injury. The overall analytical strategy for this work integrated immunoaffinity depletion of the 12 most abundant plasma proteins with cysteinyl-peptide enrichment-based fractionation prior to LC-MS analyses of individual patient samples. Incorporation of an 18O-labeled "universal" reference among the sample sets enabled precise relative quantification across samples. In total, 313 plasma proteins

SUBMITTER: Qian WJ 

PROVIDER: S-EPMC2945297 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature