Molecular species identification of Central European ground beetles (Coleoptera: Carabidae) using nuclear rDNA expansion segments and DNA barcodes.

ABSTRACT:

Background

The identification of vast numbers of unknown organisms using DNA sequences becomes more and more important in ecological and biodiversity studies. In this context, a fragment of the mitochondrial cytochrome c oxidase I (COI) gene has been proposed as standard DNA barcoding marker for the identification of organisms. Limitations of the COI barcoding approach can arise from its single-locus identification system, the effect of introgression events, incomplete lineage sorting, numts, heteroplasmy and maternal inheritance of intracellular endosymbionts. Consequently, the analysis of a supplementary nuclear marker system could be advantageous.

Results

We tested the effectiveness of the COI barcoding region and of three nuclear ribosomal expansion segments in discrimin

SUBMITTER: Raupach MJ 

PROVIDER: S-EPMC2945340 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature