A mouse model of ischemic spinal cord injury with delayed paralysis caused by aortic cross-clamping.

ABSTRACT:

Background

Spinal cord ischemia and paralysis are devastating perioperative complications that can accompany open or endovascular repair surgery for aortic aneurysms. Here, we report on the development of a new mouse model of spinal cord ischemia with delayed paralysis induced by cross-clamping the descending aorta.

Methods

Transient aortic occlusion was produced in mice by cross-clamping the descending aorta through a lateral thoracotomy. To establish an optimal surgical procedure with limited mortality, variable cross-clamp times and core temperatures were tested between experiments.

Results

The onset of paresis or paralysis and postsurgical mortality varied as a function of cross-clamp time and core temperature that was maintained during the period of cross-clamp.

SUBMITTER: Awad H 

PROVIDER: S-EPMC2945453 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature