Rat and Drosophila synaptotagmin 4 have opposite effects during SNARE-catalyzed membrane fusion.

ABSTRACT: Synaptotagmins (Syt) are a large family of proteins that regulate membrane traffic in neurons and other cell types. One isoform that has received considerable attention is SYT4, with apparently contradictory reports concerning the function of this isoform in fruit flies and mice. SYT4 was reported to function as a negative regulator of neurotrophin secretion in mouse neurons and as a positive regulator of secretion of a yet to be identified growth factor from muscle cells in flies. Here, we have directly compared the biochemical and functional properties of rat and fly SYT4. We report that rat SYT4 inhibited SNARE-catalyzed membrane fusion in both the absence and presence of Ca(2+). In marked contrast, fly SYT4 stimulated SNARE-mediated membrane fusion in response to Ca(2+). Analysis of ch

SUBMITTER: Wang Z 

PROVIDER: S-EPMC2945570 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature