Differential detection of alternatively spliced variants of Ciz1 in normal and cancer cells using a custom exon-junction microarray.

ABSTRACT:

Background

Ciz1 promotes initiation of mammalian DNA replication and is present within nuclear matrix associated DNA replication factories. Depletion of Ciz1 from normal and cancer cells restrains entry to S phase and inhibits cell proliferation. Several alternative splicing events with putative functional consequences have been identified and reported, but many more variants are predicted to exist based on publicly available mRNAs and expressed sequence tags.

Methods

Here we report the development and validation of a custom exon and exon-junction microarray focused on the human CIZ1 gene, capable of reproducible detection of differential splice-variant expression.

Results

Using a pair of paediatric cancer cell lines and a pool of eight normal lines as reference, the

SUBMITTER: Rahman FA 

PROVIDER: S-EPMC2945943 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature