T-DARPP regulates phosphatidylinositol-3-kinase-dependent cell growth in breast cancer.

ABSTRACT:

Background

Recent reports have shown that t-DARPP (truncated isoform of DARPP-32) can mediate trastuzumab resistance in breast cancer cell models. In this study, we evaluated expression of t-DARPP in human primary breast tumors, and investigated the role of t-DARPP in regulating growth and proliferation in breast cancer cells.

Results

Quantitative real time RT-PCR analysis using primers specific for t-DARPP demonstrated overexpression of t-DARPP in 36% of breast cancers (13/36) as opposed to absent to very low t-DARPP expression in normal breast tissue (p < 0.05). The mRNA overexpression of t-DARPP was overwhelmingly observed in ductal carcinomas, including invasive ductal carcinomas and intraductal carcinomas, rather than other types of breast cancers. The immunohistochemis

SUBMITTER: Vangamudi B 

PROVIDER: S-EPMC2945963 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature