An efficient multistrategy DNA decontamination procedure of PCR reagents for hypersensitive PCR applications.

ABSTRACT:

Background

PCR amplification of minute quantities of degraded DNA for ancient DNA research, forensic analyses, wildlife studies and ultrasensitive diagnostics is often hampered by contamination problems. The extent of these problems is inversely related to DNA concentration and target fragment size and concern (i) sample contamination, (ii) laboratory surface contamination, (iii) carry-over contamination, and (iv) contamination of reagents.

Methodology/principal findings

Here we performed a quantitative evaluation of current decontamination methods for these last three sources of contamination, and developed a new procedure to eliminate contaminating DNA contained in PCR reagents. We observed that most current decontamination methods are either not efficient enough to degrad

SUBMITTER: Champlot S 

PROVIDER: S-EPMC2946917 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature