QKI-7 regulates expression of interferon-related genes in human astrocyte glioma cells.

ABSTRACT:

Background

The human QKI gene, called quaking homolog, KH domain RNA binding (mouse), is a candidate gene for schizophrenia encoding an RNA-binding protein. This gene was shown to be essential for myelination in oligodendrocytes. QKI is also highly expressed in astrocytes, but its function in these cells is not known.

Methods/principal findings

We studied the effect of small interference RNA (siRNA)-mediated QKI depletion on global gene expression in human astrocyte glioma cells. Microarray measurements were confirmed with real-time quantitative polymerase chain reaction (qPCR). The presence of QKI binding sites (QRE) was assessed by a bioinformatic approach. Viability and cell morphology were also studied. The most significant alteration after QKI silencing was the decrease

SUBMITTER: Jiang L 

PROVIDER: S-EPMC2947523 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature