Gene expression changes associated with resistance to intravenous corticosteroid therapy in children with severe ulcerative colitis.

ABSTRACT:

Background and aims

Microarray analysis of RNA expression allows gross examination of pathways operative in inflammation. We aimed to determine whether genes expressed in whole blood early following initiation of intravenous corticosteroid treatment can be associated with response.

Methods

From a prospectively accrued cohort of 128 pediatric patients hospitalized for intravenous corticosteroid treatment of severe UC, we selected for analysis 20 corticosteroid responsive (hospital discharge or PUCAI ≤45 by day 5) and 20 corticosteroid resistant patients (need for second line medical therapy or colectomy, or PUCAI >45 by day 5). Total RNA was extracted from blood samples collected on day 3 of intravenous corticosteroid therapy. The eluted transcriptomes were quantified on Affy

SUBMITTER: Kabakchiev B 

PROVIDER: S-EPMC2948001 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature