Epigenetic transgenerational actions of vinclozolin on promoter regions of the sperm epigenome.

ABSTRACT: Previous observations have demonstrated that embryonic exposure to the endocrine disruptor vinclozolin during gonadal sex determination promotes transgenerational adult onset disease such as male infertility, kidney disease, prostate disease, immune abnormalities and tumor development. The current study investigates genome-wide promoter DNA methylation alterations in the sperm of F3 generation rats whose F0 generation mother was exposed to vinclozolin. A methylated DNA immunoprecipitation with methyl-cytosine antibody followed by a promoter tilling microarray (MeDIP-Chip) procedure was used to identify 52 different regions with statistically significant altered methylation in the sperm promoter epigenome. Mass spectrometry bisulfite analysis was used to map the CpG DNA methylation and 16 d

SUBMITTER: Guerrero-Bosagna C 

PROVIDER: S-EPMC2948035 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature