Functional metagenomics: a high throughput screening method to decipher microbiota-driven NF-κB modulation in the human gut.

ABSTRACT:

Background/aim

The human intestinal microbiota plays an important role in modulation of mucosal immune responses. To study interactions between intestinal epithelial cells (IECs) and commensal bacteria, a functional metagenomic approach was developed. One interest of metagenomics is to provide access to genomes of uncultured microbes. We aimed at identifying bacterial genes involved in regulation of NF-κB signaling in IECs. A high throughput cell-based screening assay allowing rapid detection of NF-κB modulation in IECs was established using the reporter-gene strategy to screen metagenomic libraries issued from the human intestinal microbiota.

Methods

A plasmid containing the secreted alkaline phosphatase (SEAP) gene under the control of NF-κB binding elements was stably tra

SUBMITTER: Lakhdari O 

PROVIDER: S-EPMC2948039 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature