Identification of differentially expressed genes in the kidneys of growth hormone transgenic mice.

ABSTRACT:

Objective

Bovine growth hormone (bGH) transgenic mice develop severe kidney damage. This damage may be due, at least in part, to changes in gene expression. Identification of genes with altered expression in the bGH kidney may identify mechanisms leading to damage in this system that may also be relevant to other models of kidney damage.

Design

cDNA subtraction libraries, northern blot analyses, microarray analyses and real-time reverse transcription polymerase chain reaction (RT/PCR) assays were used to identify and verify specific genes exhibiting differential RNA expression between kidneys of bGH mice and their non-transgenic (NT) littermates.

Results

Immunoglobulins were the vast majority of genes identified by the cDNA subtractions and the microarray analyses as

SUBMITTER: Coschigano KT 

PROVIDER: S-EPMC2948615 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature