Identification of IgE sequential epitopes of lentil (Len c 1) by means of peptide microarray immunoassay.

ABSTRACT:

Background

Lentils are often responsible for allergic reactions to legumes in Mediterranean children. Although the primary sequence of the major allergen Len c 1 is known, the location of the IgE-binding epitopes remains undefined.

Objective

We sought to identify IgE-binding epitopes of Len c 1 and relate epitope binding to clinical characteristics.

Methods

One hundred thirty-five peptides corresponding to the primary sequence of Len c 1 were probed with sera from 33 patients with lentil allergy and 15 nonatopic control subjects by means of microarray immunoassay. Lentil-specific IgE levels, skin prick test responses, and clinical reactions to lentil were determined. Epitopes were defined as overlapping signal above interslide and intraslide cutoffs and confirmed by u

SUBMITTER: Vereda A 

PROVIDER: S-EPMC2948849 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature