A simplified approach to human islet quality assessment.

ABSTRACT:

Background

The successful treatment of patients with type 1 diabetes by islet transplantation is affected by a multitude of factors of which infusion of the highest quality tissue is essential. The current standard pretransplant quality assessments lack sensitivity, accuracy, and objectivity in the determination of islet viability and potency. We hypothesized that a multiparametric approach focused on islet cell metabolic state, mitochondrial integrity, and in vitro glucose-stimulated insulin secretion (GSIS) could provide data predictive of in vivo function. The objective of this study was to validate a novel set of islet quality assays and develop a simplified islet quality scoring system for both basic research and clinical applications.

Methods

A series of 42 human islet

SUBMITTER: Hanson MS 

PROVIDER: S-EPMC2948961 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature