Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces.

ABSTRACT: Realization of the full potential of human pluripotent stem cells (hPSCs) in regenerative medicine requires the development of well-defined culture conditions for their long-term growth and directed differentiation. Current practices for maintaining hPSCs generally utilize empirically determined combinations of feeder cells and other animal-based products, which are expensive, difficult to isolate, subject to batch-to-batch variations, and unsuitable for cell-based therapies. Using a high-throughput screening approach, we identified several polymers that can support self-renewal of hPSCs. While most of these polymers provide support for only a short period of time, we identified a synthetic polymer poly(methyl vinyl ether-alt-maleic anhydride) (PMVE-alt-MA) that supported the long-term att

SUBMITTER: Brafman DA 

PROVIDER: S-EPMC2949524 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature