Genome-wide search identifies Ccnd2 as a direct transcriptional target of Elf5 in mouse mammary gland.

ABSTRACT:

Background

The ETS transcription factor Elf5 (also known as ESE-2) is highly expressed in the mammary gland and plays an important role in its development and differentiation. Indeed studies in mice have illustrated an essential role for Elf5 in directing alveologenesis during pregnancy. Although the molecular mechanisms that underlie the developmental block in Elf5 null mammary glands are beginning to be unraveled, this investigation has been hampered by limited information about the identity of Elf5-target genes. To address this shortcoming, in this study we have performed ChIP-cloning experiments to identify the specific genomic segments that are occupied by Elf5 in pregnant mouse mammary glands.

Results

Sequencing and genomic localization of cis-regulatory regions bound

SUBMITTER: Escamilla-Hernandez R 

PROVIDER: S-EPMC2949602 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature