High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library.

ABSTRACT:

Background

During the last two decades, DNA sequencing has led to the identification of numerous genes in key species; however, in most cases, their functions are still unknown. In this situation, reverse genetics is the most suitable method to assign function to a gene. TILLING (Targeting Induced Local Lesions IN Genomes) is a reverse-genetic strategy that combines random chemical mutagenesis with high-throughput discovery of the induced mutations in target genes. The method has been applied to a variety of plant and animal species. Screening of the induced mutations is the most important step in TILLING. Currently, direct sequencing or nuclease-mediated screening of heteroduplexes is widely used for detection of mutations in TILLING. Both methods are useful, but the costs are sub

SUBMITTER: Ishikawa T 

PROVIDER: S-EPMC2949603 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature