The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.

ABSTRACT:

Background

Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be thought of as the catalytic core of Mcm2-7, the replicative helicase in eukaryotic cells. Oligomeric analysis of Mcm4/6/7 suggests that it forms a hexamer containing two Mcm4/6/7 trimers, however, under certain conditions trimeric Mcm4/6/7 has also been observed. The functional significance of the different Mcm4/6/7 oligomeric states has not been assessed. The results of such an assessment would have implications for studies of both Mcm4/6/7 and Mcm2-7.

Results

Here, we show that Saccharomyces cerevisiae Mcm4/6/7 reconstituted from individu

SUBMITTER: Ma X 

PROVIDER: S-EPMC2949612 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature