A high-throughput method for isolation of salicylic acid metabolic mutants.

ABSTRACT:

Background

Salicylic acid (SA) is a key defense signal molecule against biotrophic pathogens in plants. Quantification of SA levels in plants is critical for dissecting the SA-mediated immune response. Although HPLC and GC/MS are routinely used to determine SA concentrations, they are expensive and time-consuming. We recently described a rapid method for a bacterial biosensor Acinetobacter sp. ADPWH_lux-based SA quantification, which enables high-throughput analysis. In this study we describe an improved method for fast sample preparation, and present a high-throughput strategy for isolation of SA metabolic mutants.

Results

On the basis of the previously described biosensor-based method, we simplified the tissue collection and the SA extraction procedure. Leaf discs were col

SUBMITTER: Marek G 

PROVIDER: S-EPMC2949671 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature