Effects of osteopontin inhibition on radiosensitivity of MDA-MB-231 breast cancer cells.

ABSTRACT:

Background

Osteopontin (OPN) is a secreted glycophosphoprotein that is overexpressed in various tumors, and high levels of OPN have been associated with poor prognosis of cancer patients. In patients with head and neck cancer, high OPN plasma levels have been associated with poor prognosis following radiotherapy. Since little is known about the relationship between OPN expression and radiosensitivity, we investigated the cellular and radiation induced effects of OPN siRNA in human MDA-MB-231 breast cancer cells.

Methods

MDA-MB-231 cells were transfected with OPN-specific siRNAs and irradiated after 24 h. To verify the OPN knockdown, we measured the OPN mRNA and protein levels using qRT-PCR and Western blot analysis. Furthermore, the functional effects of OPN siRNAs were stud

SUBMITTER: Hahnel A 

PROVIDER: S-EPMC2949679 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature