Membrane proteomic analysis of pancreatic cancer cells.

ABSTRACT:

Background

Pancreatic cancer is one of the most aggressive human tumors due to its high potential of local invasion and metastasis. The aim of this study was to characterize the membrane proteomes of pancreatic ductal adenocarcinoma (PDAC) cells of primary and metastatic origins, and to identify potential target proteins related to metastasis of pancreatic cancer.

Methods

Membrane/membrane-associated proteins were isolated from AsPC-1 and BxPC-3 cells and identified with a proteomic approach based on SDS-PAGE, in-gel tryptic digestion and liquid chromatography with tandem mass spectrometry (LC-MS/MS). X! Tandem was used for database searching against the SwissProt human protein database.

Results

We identified 221 & 208 proteins from AsPC-1 and BxPC-3 cells, respective

SUBMITTER: Liu X 

PROVIDER: S-EPMC2949717 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature