TcTASV: a novel protein family in trypanosoma cruzi identified from a subtractive trypomastigote cDNA library.

ABSTRACT:

Background

The identification and characterization of antigens expressed in Trypanosoma cruzi stages that parasitize mammals are essential steps for the development of new vaccines and diagnostics. Genes that are preferentially expressed in trypomastigotes may be involved in key processes that define the biology of trypomastigotes, like cell invasion and immune system evasion.

Methodology/principal findings

With the initial aim of identifying trypomastigote-specific expressed tags, we constructed and sequenced an epimastigote-subtracted trypomastigote cDNA library (library TcT-E). More than 45% of the sequenced clones of the library could not be mapped to previously annotated mRNAs or proteins. We validated the presence of these transcripts by reverse northern blot and north

SUBMITTER: Garcia EA 

PROVIDER: S-EPMC2950142 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature