Heterologous expression and extracellular secretion of cellulolytic enzymes by Zymomonas mobilis.

ABSTRACT: Development of the strategy known as consolidated bioprocessing (CBP) involves the use of a single microorganism to convert pretreated lignocellulosic biomass to ethanol through the simultaneous production of saccharolytic enzymes and fermentation of the liberated monomeric sugars. In this report, the initial steps toward achieving this goal in the fermentation host Zymomonas mobilis were investigated by expressing heterologous cellulases and subsequently examining the potential to secrete these cellulases extracellularly. Numerous strains of Z. mobilis were found to possess endogenous extracellular activities against carboxymethyl cellulose, suggesting that this microorganism may harbor a favorable environment for the production of additional cellulolytic enzymes. The heterologous express

SUBMITTER: Linger JG 

PROVIDER: S-EPMC2950452 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature