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A targeted multilocus genotyping assay for lineage, serogroup, and epidemic clone typing of Listeria monocytogenes.


ABSTRACT: A 30-probe assay was developed for simultaneous classification of Listeria monocytogenes isolates by lineage (I to IV), major serogroup (4b, 1/2b, 1/2a, and 1/2c), and epidemic clone (EC) type (ECI, ECIa, ECII, and ECIII). The assay was designed to facilitate rapid strain characterization and the integration of subtype data into risk-based inspection programs.

SUBMITTER: Ward TJ 

PROVIDER: S-EPMC2950480 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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A targeted multilocus genotyping assay for lineage, serogroup, and epidemic clone typing of Listeria monocytogenes.

Ward Todd J TJ   Usgaard Thomas T   Evans Peter P  

Applied and environmental microbiology 20100813 19


A 30-probe assay was developed for simultaneous classification of Listeria monocytogenes isolates by lineage (I to IV), major serogroup (4b, 1/2b, 1/2a, and 1/2c), and epidemic clone (EC) type (ECI, ECIa, ECII, and ECIII). The assay was designed to facilitate rapid strain characterization and the integration of subtype data into risk-based inspection programs. ...[more]

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