Comparative epigenomic analysis of murine and human adipogenesis.

ABSTRACT: We report the generation and comparative analysis of genome-wide chromatin state maps, PPARγ and CTCF localization maps, and gene expression profiles from murine and human models of adipogenesis. The data provide high-resolution views of chromatin remodeling during cellular differentiation and allow identification of thousands of putative preadipocyte- and adipocyte-specific cis-regulatory elements based on dynamic chromatin signatures. We find that the specific locations of most such elements differ between the two models, including at orthologous loci with similar expression patterns. Based on sequence analysis and reporter assays, we show that these differences are determined, in part, by evolutionary turnover of transcription factor motifs in the genome sequences and that this turnover

SUBMITTER: Mikkelsen TS 

PROVIDER: S-EPMC2950833 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature