Genotyping the BDNF rs6265 (val66met) polymorphism by one-step amplified refractory mutation system PCR.

ABSTRACT:

Background

The brain derived neutrophic factor (BDNF), a 27 kD polypeptide, is one of the most widely expressed neurotrophins in the brain, regulating neural development and plasticity. The BDNF gene contains a functional single-nucleotide polymorphism (rs6265), which results in a valine to methionine substitution (val66met), leading to reduced mature BDNF expression. This polymorphism has been widely implicated in a host of psychiatric disorders and is a focus of many ongoing psychiatric genetic studies.

Objective

To develop an efficient and rapid method to detect the val66met polymorphism in a one-step PCR reaction.

Method and results

We have designed four PCR primers that amplify the BDNF gene region containing rs6265. The specificity of the four primers in a singl

SUBMITTER: Sheikh HI 

PROVIDER: S-EPMC2950863 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature