ATP control of dynamic P1 ParA-DNA interactions: a key role for the nucleoid in plasmid partition.

ABSTRACT: P1 ParA is a member of the Walker-type family of partition ATPases involved in the segregation of plasmids and bacterial chromosomes. ATPases of this class interact with DNA non-specifically in vitro and colocalize with the bacterial nucleoid to generate a variety of reported patterns in vivo. Here, we directly visualize ParA binding to DNA using total internal reflection fluorescence microscopy. This activity depends on, and is highly specific for ATP. DNA-binding activity is not coupled to ATP hydrolysis. Rather, ParA undergoes a slow multi-step conformational transition upon ATP binding, which licenses ParA to bind non-specific DNA. The kinetics provide a time-delay switch to allow slow cycling between the DNA binding and non-binding forms of ParA. We propose that this time delay, combi

SUBMITTER: Vecchiarelli AG 

PROVIDER: S-EPMC2950902 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature