Isolation of murine bone marrow derived mesenchymal stem cells using Twist2 Cre transgenic mice.

ABSTRACT: While human bone marrow derived mesenchymal stem cells (BMSCs) are of great interest for their potential therapeutic value, their murine equivalent remains an important basic research model that can provide critical insights into the biology of this progenitor cell population. Here we present a novel transgenic strategy that allowed for the selective identification and isolation of murine BMSCs at the early stages of stromal cell culture. This strategy involved crossing Twist2 -Cre mice with Cre reporter mice such as Z/EG or Ai9, which express EGFP or Tomato fluorescent protein, respectively, upon Cre mediated excision of a stop sequence. Using this approach, we identified an adherent fluorescent protein+cell population (T2C+) that is present during the earliest stages of colony formation

SUBMITTER: Liu Y 

PROVIDER: S-EPMC2952694 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature