Gene expression profiling reveals distinct cocaine-responsive genes in human fetal CNS cell types.

ABSTRACT:

Objectives

Prenatal exposure to cocaine causes cytoarchitectural alterations in the developing neocortex. Previously, we reported that cocaine inhibits neural progenitor cell proliferation through oxidative endoplasmic reticulum stress and consequent down-regulation of cyclin A, whereas cyclin A expression was increased in astrocytes. In the present study, cell type-specific responses to cocaine were further explored.

Methods

Gene expression profiles were examined in five types of cells obtained from the human fetal cerebral cortex at 20 weeks gestation. Cells were treated with 100 µM cocaine in vitro for 24 hr, followed by gene expression analysis using a human neural/stem cell/drug abuse-focused cDNA array, with verification by quantitative real-time RT-PCR.

Results

SUBMITTER: Lee CT 

PROVIDER: S-EPMC2952961 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature