Simultaneous genotyping and quantification of hepatitis B virus for genotypes B and C by real-time PCR assay.

ABSTRACT: Hepatitis B virus (HBV) is an important cause of human chronic liver diseases and is a major public health problem. Viral load and HBV genotype play critical roles in determining clinical outcomes and response to antiviral treatment in hepatitis B patients. Viral genotype detection and quantification assays are currently in use with different levels of effectiveness. In this study, the performance of a real-time genotyping and quantitative PCR (GQ-PCR)-based assay was evaluated. Through the use of genotype-specific primers and probes, this assay provides simultaneous identification and quantification of genotypes B and C in a single reaction. Our GQ-PCR correctly identified all predefined genotypes B and C, and no cross-reaction between genotypes B and C were observed. The GQ-PCR identifie

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC2953110 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature