MicroRNA discovery and analysis of pinewood nematode Bursaphelenchus xylophilus by deep sequencing.

ABSTRACT:

Background

MicroRNAs (miRNAs) are considered to be very important in regulating the growth, development, behavior and stress response in animals and plants in post-transcriptional gene regulation. Pinewood nematode, Bursaphelenchus xylophilus, is an important invasive plant parasitic nematode in Asia. To have a comprehensive knowledge about miRNAs of the nematode is necessary for further in-depth study on roles of miRNAs in the ecological adaptation of the invasive species.

Methods and findings

Five small RNA libraries were constructed and sequenced by Illumina/Solexa deep-sequencing technology. A total of 810 miRNA candidates (49 conserved and 761 novel) were predicted by a computational pipeline, of which 57 miRNAs (20 conserved and 37 novel) encoded by 53 miRNA precursors

SUBMITTER: Huang QX 

PROVIDER: S-EPMC2953492 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature