Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications.

ABSTRACT:

Background

A large portion of tissues stored worldwide for diagnostic purposes is formalin-fixed and paraffin-embedded (FFPE). These FFPE-archived tissues are an extremely valuable source for retrospective (genetic) studies. These include mutation screening in cancer-critical genes as well as pathogen detection. In this study we evaluated the impact of several widely used DNA extraction methods on the quality of molecular diagnostics on FFPE tissues.

Findings

We compared 4 DNA extraction methods from 4 identically processed FFPE mammary-, prostate-, colon- and lung tissues with regard to PCR inhibition, real time SNP detection and amplifiable fragment size. The extraction methods, with and without proteinase K pre-treatment, tested were: 1) heat-treatment, 2) QIAamp DNA-bloo

SUBMITTER: Huijsmans CJ 

PROVIDER: S-EPMC2954845 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature