Multispectral fingerprinting for improved in vivo cell dynamics analysis.

ABSTRACT:

Background

Tracing cell dynamics in the embryo becomes tremendously difficult when cell trajectories cross in space and time and tissue density obscure individual cell borders. Here, we used the chick neural crest (NC) as a model to test multicolor cell labeling and multispectral confocal imaging strategies to overcome these roadblocks.

Results

We found that multicolor nuclear cell labeling and multispectral imaging led to improved resolution of in vivo NC cell identification by providing a unique spectral identity for each cell. NC cell spectral identity allowed for more accurate cell tracking and was consistent during short term time-lapse imaging sessions. Computer model simulations predicted significantly better object counting for increasing cell densities in 3-color co

SUBMITTER: Kulesa PM 

PROVIDER: S-EPMC2954993 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature